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Author Femi-Ola, Titilayo Olufunke
Affiliation Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria
Author Orjiakor, Paul Ikechukwu
Affiliation Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria
Author Enerijiofi, Kingsley Erhons
Affiliation Samuel Adegboyega University, College of Basic and Applied Sciences Department of Biological Sciences, Ogwa, Edo State, Nigeria
E-mail paulorjiakor@gmail.com
Author Oke, Iyanuoluwa Omolade
Affiliation Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria
Author Fatoyinbo, Adebayo Adeola
Affiliation Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria
ISSN printed 1733-8670
URI https://repository.scientific-journals.eu/handle/123456789/2542
Abstract Soil contaminated with crude oil has negatively affected some of the communities in Ekiti State region in Nigeria. There is an increased interest in microbial lipases because of their enormous potential in industrial and biotechnological applications. This study investigated the characteristics of partially-purified lipase from Pseudomonas aeruginosa isolated from oil-contaminated soil in Ado-Ekiti, Ekiti State. The lipase was purified by gel-filtration, having a molecular weight of 39.11 kDa, with Km and Vmax values of 12.50 and 28.86, respectively. The enzyme had an optimal pH of 8.0 and exhibited its maximal activity at 50°C, whereas the relatively stable temperature and pH were 40°C and 5.0, respectively. The enzyme activity was enhanced by olive oil, which served as the carbon source. Sodium chloride enhanced lipase activity, while calcium chloride acted as mild inhibitor, and iron chloride acted a strong inhibitor. The lipase from Pseudomonas aeruginosa possessed properties of an industrial enzyme and will be useful for biodegradation and bioremediation studies
Pages 154-161
Publisher Scientific Journals Maritime University of Szczecin, Zeszyty Naukowe Akademia Morska w Szczecinie
Keywords biodegradation
Keywords enzymes
Keywords gel filtration
Keywords lipase
Keywords olive oil
Keywords Pseudomonas aeruginosa
Title Kinetic properties of Lipase obtained from Pseudomonas aeruginosa isolated from crude oil contaminated soil
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ISSN on-line 2392-0378
Language English
Funding No data
Figures 9
Tables 1
DOI 10.17402/363
Published 2019-10-04
Accepted 2019-07-29
Recieved 2019-04-04


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